Microscopic identification of novel cell types in the integument of larval lake sturgeon, Acipenser fulvescens.
Identifieur interne : 000050 ( Main/Exploration ); précédent : 000049; suivant : 000051Microscopic identification of novel cell types in the integument of larval lake sturgeon, Acipenser fulvescens.
Auteurs : Lauren Shute [Canada] ; Erwin Huebner [Canada] ; W Gary Anderson [Canada]Source :
- Journal of morphology [ 1097-4687 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- anatomy & histology : Fishes, Integumentary System.
- cytology : Larva.
- Animals.
Abstract
Osmoregulation, respiration, nutrient/mineral transport, and defense mechanisms are all evident in the integument of fish. The role of the integument in these physiological processes is particularly important during early life history in larval fishes, as functional systems such as the gills and gastrointestinal tract are not fully developed. Using a variety of microscopy techniques, we describe the morphology of keratinocytes, mitochondria rich cells, ciliated cells and mucous cells of the skin, yolk sac, and gills. The cytology we observed was similar to previous studies describing the integument of larval fish, however, we have also identified two novel cell types on the integument of larval Lake Sturgeon, Acipenser fulvescens, between 9 and 34 days post fertilization. Our detailed analysis included a multifaceted microscopy approach using scanning electron, transmission electron, and light microscopy to elucidate the histology of the tissue and cellular morphology in addition to quantification and distribution of these novel cell types. The first cell type had a characteristic ampullary shape with a central cavity and a pore opening at the surface. The second, located on the free surface of the epidermis, had an uneven plasma membrane surface. Based on the abundance of secretory vesicles, organelles necessary for protein synthesis, and the lack of neural connection in both cell types, we propose these cells to be involved in the release of semiochemicals that may act as a pheromone, alarm substance, or chemical defense mechanism.
DOI: 10.1002/jmor.20480
PubMed: 26440535
Affiliations:
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Le document en format XML
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Shute</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, University of Manitoba, Winnipeg, Canada, Manitoba, R3T 2N2.</nlm:affiliation><orgName type="university">Université du Manitoba</orgName><country>Canada</country><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Huebner, Erwin" sort="Huebner, Erwin" uniqKey="Huebner E" first="Erwin" last="Huebner">Erwin Huebner</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, University of Manitoba, Winnipeg, Canada, Manitoba, R3T 2N2.</nlm:affiliation><orgName type="university">Université du Manitoba</orgName><country>Canada</country><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, University of Manitoba, Winnipeg, Canada, Manitoba, R3T 2N2.</nlm:affiliation><orgName type="university">Université du Manitoba</orgName><country>Canada</country><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author></analytic><series><title level="j">Journal of morphology</title><idno type="eISSN">1097-4687</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Fishes (anatomy & histology)</term><term>Integumentary System (anatomy & histology)</term><term>Larva (cytology)</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Fishes</term><term>Integumentary System</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Larva</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Osmoregulation, respiration, nutrient/mineral transport, and defense mechanisms are all evident in the integument of fish. The role of the integument in these physiological processes is particularly important during early life history in larval fishes, as functional systems such as the gills and gastrointestinal tract are not fully developed. Using a variety of microscopy techniques, we describe the morphology of keratinocytes, mitochondria rich cells, ciliated cells and mucous cells of the skin, yolk sac, and gills. The cytology we observed was similar to previous studies describing the integument of larval fish, however, we have also identified two novel cell types on the integument of larval Lake Sturgeon, Acipenser fulvescens, between 9 and 34 days post fertilization. Our detailed analysis included a multifaceted microscopy approach using scanning electron, transmission electron, and light microscopy to elucidate the histology of the tissue and cellular morphology in addition to quantification and distribution of these novel cell types. The first cell type had a characteristic ampullary shape with a central cavity and a pore opening at the surface. The second, located on the free surface of the epidermis, had an uneven plasma membrane surface. Based on the abundance of secretory vesicles, organelles necessary for protein synthesis, and the lack of neural connection in both cell types, we propose these cells to be involved in the release of semiochemicals that may act as a pheromone, alarm substance, or chemical defense mechanism.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="Canada"><region name="Manitoba"><name sortKey="Shute, Lauren" sort="Shute, Lauren" uniqKey="Shute L" first="Lauren" last="Shute">Lauren Shute</name></region><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name><name sortKey="Huebner, Erwin" sort="Huebner, Erwin" uniqKey="Huebner E" first="Erwin" last="Huebner">Erwin Huebner</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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